| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.013604 |
| Chromosome: | chromosome 10 |
| Location: | 5218358 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456000 | FAP191 | (1 of 4) K03809 - NAD(P)H dehydrogenase (quinone) (wrbA); Flagellar Associated Protein191 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCAGCGCCCAAGACGGAGGCTGCTAAGCCGGTGGCGGAGCCGGCCCC |
| Internal bar code: | AGCAAGTCCCATATAGCTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3674 |
| LEAP-Seq percent confirming: | 93.0233 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTGCTGGTCTCCATCAC |
| Suggested primer 2: | TTGGTATCTGGGTGGTGCAC |