| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.013614 |
| Chromosome: | chromosome 17 |
| Location: | 1784551 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708950 | (1 of 2) 3.2.1.58 - Glucan 1,3-beta-glucosidase / Exo-1,3-beta-glucosidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAACCCACCTTCCCCGGCGCCACCCAGCCCACAGCCGCCATCACCGGC |
| Internal bar code: | GCTGTATTGGAGTTAGCAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2048 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCGGGGTTGATGTAAGAC |
| Suggested primer 2: | TGACAACTCGCGCAAAAAGG |