| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.013617 |
| Chromosome: | chromosome 9 |
| Location: | 3006042 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393543 | HCP2 | (1 of 4) 1.7.99.1 - Hydroxylamine reductase / Hydroxylamine (acceptor) reductase; Hybrid-cluster protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCACGTCCTTGGCGCCGGGCGTGTTCAGGAAGTGCAGCGCCCCCTGC |
| Internal bar code: | TACGTGTTTTGCAGACTCGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3154 |
| LEAP-Seq percent confirming: | 85.4167 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACCCTCGACCCATATGC |
| Suggested primer 2: | ACATCCTTCTTCTTGGGCCG |