| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013629 |
| Chromosome: | chromosome 4 |
| Location: | 3714884 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229163 | (1 of 1) PF00169//PF02181 - PH domain (PH) // Formin Homology 2 Domain (FH2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGAATGTAGCCCCCTCTCCCCGCATCCCCTAGAACTTCACTTCTTCTT |
| Internal bar code: | GTTGGCTTCACTTAATGGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 53 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGTACGCAGCTAGGCAC |
| Suggested primer 2: | CCAAAGCCAACGAAAAGGGG |