| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.013665 |
| Chromosome: | chromosome 13 |
| Location: | 2506794 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579950 | (1 of 1) PTHR12760:SF0 - ER MEMBRANE PROTEIN COMPLEX SUBUNIT 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAATGGTTGCGATCTGGCTCCCGTCGACGAGCGGCGCAACAAGCACA |
| Internal bar code: | GTCCTTGGGGATGAAGGGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCGGTACATCTCCTCTG |
| Suggested primer 2: | CCACTACAAGTCGCACCTGT |