Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.013673 |
Chromosome: | chromosome 10 |
Location: | 1697928 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g429750 | FAP417 | (1 of 1) IPR000014//IPR000104 - PAS domain // Antifreeze protein, type I; Flagellar Associated Protein 417 | 3'UTR |
Cre10.g429800 | COQ8,ABC1 | Ubiquinone biosynthesis protein; (1 of 1) PTHR10566:SF65 - UBIQUINONE BIOSYNTHESIS PROTEIN COQ-8 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGGCTGTTTTGTATGAGCGTGCGGTTGTGTGGTGACGGGGGCACACC |
Internal bar code: | AATCGAAAATCATCCAGGTCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 378 |
LEAP-Seq percent confirming: | 20.0 |
LEAP-Seq n confirming: | 1 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACGGCATGAAACCCCCTTC |
Suggested primer 2: | CCCCACCACCACAGATAACC |