Insertion junction: CLIP2.013673_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.013673
Chromosome:chromosome 10
Location:1697928
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre10.g429750 FAP417 (1 of 1) IPR000014//IPR000104 - PAS domain // Antifreeze protein, type I; Flagellar Associated Protein 417 3'UTR
Cre10.g429800 COQ8,ABC1 Ubiquinone biosynthesis protein; (1 of 1) PTHR10566:SF65 - UBIQUINONE BIOSYNTHESIS PROTEIN COQ-8 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGGCTGTTTTGTATGAGCGTGCGGTTGTGTGGTGACGGGGGCACACC
Internal bar code:AATCGAAAATCATCCAGGTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:378
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TACGGCATGAAACCCCCTTC
Suggested primer 2:CCCCACCACCACAGATAACC