| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013679 |
| Chromosome: | chromosome 14 |
| Location: | 1559863 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618150 | (1 of 1) PF07058 - Microtubule-associated protein 70 (MAP70) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTAGAGCTCCCTGTGCACAGGGCTAACACAACCTGGGCCCACCATTC |
| Internal bar code: | ATGTGATGTGGTTTACTAACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4730 |
| LEAP-Seq percent confirming: | 90.2857 |
| LEAP-Seq n confirming: | 158 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 175 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCGCATCTTGAAGGAGC |
| Suggested primer 2: | TTCTCGCCGTCCATCTTAGC |