Insertion junction: CLIP2.013703_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.013703
Chromosome:chromosome 13
Location:3850050
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGGGATCGGCTTCTCACCGCCCTCGACGAACACGCCACAAGGCGGTAG
Internal bar code:GACCTCCTTGGGGGCTTTATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:525
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AAAAACGTTCTCGCACACGG
Suggested primer 2:GGAGAGGACGTAGGTCACCT