Insertion junction: CLIP2.013707_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.013707
Chromosome:chromosome 10
Location:5722237
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g459550 B9D2B (1 of 1) PTHR12968:SF2 - B9 DOMAIN-CONTAINING PROTEIN 2; B9 Domain-Containing transition zone protein 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTGTCTGGCCGGACGCTGTTACTCACGGACCCTGCGCGTGCTCGCCCT
Internal bar code:CTCGGCTCAAGGGGGTGTCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:4707
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:72

Suggested primers for confirmation by PCR

Suggested primer 1:CAAACCGAAAGACGCTTGCA
Suggested primer 2:AGTCCCGCACCTTCTTGAAG