| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013708 |
| Chromosome: | chromosome 10 |
| Location: | 1449840 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428150 | (1 of 1) PTHR10566:SF58 - PROTEIN D2023.6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGGGAGGTGGGGATTACATTCATGGTTGATGACGAAGAAGCGGGAG |
| Internal bar code: | TACTCGCTATCAGATACTTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1542 |
| LEAP-Seq percent confirming: | 87.8049 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCTCTACTGCATGCAA |
| Suggested primer 2: | TGCTACATGTGTGCCGTTCT |