Insertion junction: CLIP2.013716_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.013716
Chromosome:chromosome 2
Location:2852018
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g094700 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAACGCCACGCATTGAAGTCACATTGGCCCCACTCTGCTGCCTTCATC
Internal bar code:GAAGTCGGAATTAGAGACCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:4291
LEAP-Seq percent confirming:98.7342
LEAP-Seq n confirming:78
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:79

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGTGCACCATGATGATC
Suggested primer 2:TGTACTGTACACACACGCCC