| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.013718 |
| Chromosome: | chromosome 6 |
| Location: | 3046680 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274700 | OTU4 | (1 of 2) K13719 - ubiquitin thioesterase OTU1 (OTU1, YOD1); OTU-like cysteine protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGATGCGGGCGAATGCTCAATGGTGGCGTACTGGCGTGGCTGTGCGGT |
| Internal bar code: | TAAGCTATATTTGTTGTGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2088 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGGTGCGGGAAATCTGT |
| Suggested primer 2: | GACTCGCGTGTGTTTTGCTT |