| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.013722 |
| Chromosome: | chromosome 6 |
| Location: | 1810142 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263450 | EEF1A3 | (1 of 3) K03231 - elongation factor 1-alpha (EEF1A); Putative eukaryotic translation initiation factor 1 alpha | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCGACGGAAATGGCTGGTTGCTGATGCTGCTTTGCGCGGGGCTGCCAA |
| Internal bar code: | TTCGTGAGACATGAGTCCCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1160 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAGACGACCTCAGCCATC |
| Suggested primer 2: | AGTTCTTCACTGACCGCTGG |