Insertion junction: CLIP2.013725_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.013725
Chromosome:chromosome 4
Location:1345401
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g214501 PNP1,PNT2 Chloroplast polyribonucleotide phosphorylase; (1 of 1) 2.7.7.8 - Polyribonucleotide nucleotidyltransferase / Polynucleotide phosphorylase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAATTTCGGAAGTGATTGCAAGGTTAAATTGGTTGTTGATTGTTGCGG
Internal bar code:CTAGGGACAGTAATCCAATCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:321
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTACACGCACACACACACAC
Suggested primer 2:GCTCTCTACGCCTTCATCCC