| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.013778 |
| Chromosome: | chromosome 12 |
| Location: | 3085899 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501100 | TMU3,TRM2A | (1 of 1) 2.1.1.189//2.1.1.190 - 23S rRNA (uracil(747)-C(5))-methyltransferase // 23S rRNA (uracil(1939)-C(5))-methyltransferase / RNA uridine methyltransferase A; tRNA (uracil-5-)-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGCGGCGTCGTTCTTCCAGACCAACACGGCGCAGGCGGCGGTGCTGT |
| Internal bar code: | CTGCGTGTTATCGCTGCCTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCAAAGACGACCGCATC |
| Suggested primer 2: | CCAAGCCCGAGTACATGGTT |