| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.013783 |
| Chromosome: | chromosome 12 |
| Location: | 3408936 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497800 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTTGGCCGGCCCACGTCGCCTGGCGCGGGCGCGTTGCCGCCGCCGAC |
| Internal bar code: | TTTTGTGCACGGATGAGAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3448 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGCTGGTTTCGCTAAGA |
| Suggested primer 2: | CTTGCTTGTGCTTGCAGGAG |