Insertion junction: CLIP2.013784_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.013784
Chromosome:chromosome 1
Location:4050595
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g800076 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCGCGGGGCTGGACGCGGCCGCGGTGCTGGCGATGATGATGCCACTG
Internal bar code:TTTGGTTATGCGAATCTCAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:2144
LEAP-Seq percent confirming:56.25
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCACAACGCATGGCAACT
Suggested primer 2:AGGAAACTTCGATGCTGCCA