| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.013786 |
| Chromosome: | chromosome 16 |
| Location: | 874946 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647901 | CSC8 | Chlamydomonadale-specific family C protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTCCAAAGAATGCTGCAAAGCATGACGTCAATAAATCGCGGAATGAA |
| Internal bar code: | AAAGAGCCGGGCATTGGATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2104 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACGCGCTAATCGAACAG |
| Suggested primer 2: | ATTGTTCCCCTCGTTCGGTC |