Insertion junction: CLIP2.013816_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.013816
Chromosome:chromosome 11
Location:2357172
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095129 (1 of 1) PTHR23257//PTHR23257:SF537 - SERINE-THREONINE PROTEIN KINASE // PROTEIN KINASE FAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCATGGCGGGGTGCGTCAGGCACAGCGGCAGTCGTTGGGGACGGGACG
Internal bar code:CATCTTATAGGACTTTCGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1540
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:GTAACCATCCGTGAAGGGCA
Suggested primer 2:CTTCAACCACACAAGCACCG