| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.013826 |
| Chromosome: | chromosome 12 |
| Location: | 1677014 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486400 | (1 of 1) PTHR22957//PTHR22957:SF26 - TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN // PROTEIN TBC-3, ISOFORM B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACACACGAGCGATAGGCAGGTCCGGCCCGTCCTCGCTTTGCTTGCGGG |
| Internal bar code: | GGGGCTGAACCCTGTCTTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2544 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAACGCAACACTGTCCTG |
| Suggested primer 2: | GCTGTACCCGAACCAACTGA |