| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013839 |
| Chromosome: | chromosome 12 |
| Location: | 8759829 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547300 | (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGATGGTGGAGGGGCGAGAGCTGGTAGGAGGTTGCGCAGCTGCTGC |
| Internal bar code: | GGGTCGCATGCTTACGGCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 578 |
| LEAP-Seq percent confirming: | 7.14286 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCGGCCATCAGGACTACC |
| Suggested primer 2: | CGTGTGTGTTTGAGAGCACG |