Insertion junction: CLIP2.013842_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.013842
Chromosome:chromosome 2
Location:3970813
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g102551 CSV1 Chlamydomonas-specific family protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATCAACCAGTTGCGCGCCCCTGTCGATAACCGCATGTCACTCAGTATG
Internal bar code:GTGGTCGATTAGAGCTGAGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:204
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTCTGACCTTGGCACAGG
Suggested primer 2:AGGTAGTTCTGGCGTCATGC