| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013846 |
| Chromosome: | chromosome 2 |
| Location: | 6314220 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387500 | OPR38 | OctotricoPeptide Repeat protein 38 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGAAGTGCGCCAGCTTGCTGTGGATCTCCTGCGCCACAGCGTCCATCA |
| Internal bar code: | GTTATTTCACAGACATGAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2309 |
| LEAP-Seq percent confirming: | 81.0127 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCGTCGACGTCCACTAG |
| Suggested primer 2: | CAGTACATCACCCCACCACC |