Insertion junction: CLIP2.013862_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.013862
Chromosome:chromosome 17
Location:3345037
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g722851 (1 of 6) 1.1.1.100 - 3-oxoacyl-[acyl-carrier-protein] reductase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGGTCGCGCACCTTTGGGCCTCGCATGGCTTTCAAGTAGCGCTTCTCA
Internal bar code:GAGGATCGATCTATTTGGGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:373
LEAP-Seq percent confirming:4.34783
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGTCGTGTACGGTTGGAA
Suggested primer 2:ATCAGGCGCTACAGATCTGC