| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.013863 |
| Chromosome: | chromosome 5 |
| Location: | 2129078 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236926 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCGGTCGACAAAGTATGAGGGAATTATGTCTAAGCACAAGCGCGCACA |
| Internal bar code: | CGATGTGTCCGTTCATGGTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2467 |
| LEAP-Seq percent confirming: | 98.2857 |
| LEAP-Seq n confirming: | 172 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 175 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACACACAAGCTCCAGGC |
| Suggested primer 2: | ACCAGATGTTGAGGGCGAAG |