Insertion junction: CLIP2.013868_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.013868
Chromosome:chromosome 12
Location:9053993
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g544662 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACATCCTTGCAACCCCACCCCACCCCACGCACTCCTTAACACTAAAGC
Internal bar code:GCTTGTTGAGCTCGCATTCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2254
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CACTATCATTGTGGCGCACG
Suggested primer 2:CTTACTGGAGCGCTAGGTCG