Insertion junction: CLIP2.013878_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.013878
Chromosome:chromosome 17
Location:2605187
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g716451 (1 of 1) 1.14.11.13 - Gibberellin 2-beta-dioxygenase / Gibberellin 2-beta-hydroxylase; Similar to plant Gibberellin 2-beta-dioxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTGTTAAGTGGCTGCCCTCGTTTGGCCCGACAATGTCATTGAGAATGG
Internal bar code:TAATGGGTTATGGAATCCCACC

Confirmation - LEAP-Seq

LEAP-Seq distance:2066
LEAP-Seq percent confirming:97.2603
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTTGCGTTGTTGCTGTGT
Suggested primer 2:GTTCCCACAGGCGTAGAACA