| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.013881 |
| Chromosome: | chromosome 9 |
| Location: | 5983109 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412350 | (1 of 1) PTHR16557:SF4 - 2-OXOGLUTARATE-DEPENDENT DIOXYGENASE FAMILY PROTEIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGATCCGGAGGTGGAGGGCGCCATGTCGGGCGGACAGATGTCGTACG |
| Internal bar code: | GGCTACGGTCGTCATTCCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1672 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGTGGAGCGGATTGGTT |
| Suggested primer 2: | TGTGTGTGTGTGTGTGTGGA |