| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013883 |
| Chromosome: | chromosome 14 |
| Location: | 1871348 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619900 | PHX22,PFH22 | Putative prolyl 4-hydroxylase; (1 of 1) PTHR10869:SF80 - IRON ION BINDING / OXIDOREDUCTASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAAGCACCTGCTGCATTGCCAAAATTGCCAAAGCTGGTCGCCAAGCAC |
| Internal bar code: | TTAAGCGGCTAGCCTAAGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 482 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGTTTGTTGCAGCAGAG |
| Suggested primer 2: | GGGATGTGGGGTGTTACAGG |