Insertion junction: CLIP2.013897_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.013897
Chromosome:chromosome 2
Location:7185397
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTATATTGATTGGGCTAGGTTGTGCGCGGATTGCCCCGATCGACTTAACT
Internal bar code:CAAAGAGCATTGTGAAGAGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1754
LEAP-Seq percent confirming:88.2353
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GCATGAAGGGGGAGCTCTTT
Suggested primer 2:ACAAACAATGACACTGCGGC