| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.013901 |
| Chromosome: | chromosome 3 |
| Location: | 123296 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g143847 | (1 of 3) PF07386 - Protein of unknown function (DUF1499) (DUF1499) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATTGACTCCTTCCTGCCATGTATCTTCAGATTGCTCCACGGCCCTTTC |
| Internal bar code: | GCTGATCGGCCAATGCCGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1548 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCTTTGCACTTGCTCTG |
| Suggested primer 2: | CGGAGTACCACGCCACTATC |