Insertion junction: CLIP2.013904_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.013904
Chromosome:chromosome 6
Location:2574387
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g269700 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGGTCTATACTGCGCCTCACCGTACACGCATTTCTATCGGGCACCGC
Internal bar code:TTCTGGCGGTCACCTGGGGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:448
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GCACACAACAGTCAACACCC
Suggested primer 2:CTGGTGGTGGTTGTCAGGTT