| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.013916 |
| Chromosome: | chromosome 7 |
| Location: | 4580035 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344150 | (1 of 1) PTHR21314//PTHR21314:SF0 - UNCHARACTERIZED // UPF0553 PROTEIN C9ORF64 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAGGAAACTCGTGGAGTCGTTAGATTGAAATCCGGCGCGTGAGTGCTA |
| Internal bar code: | GCCTTGATTTAACAAATACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1575 |
| LEAP-Seq percent confirming: | 76.9231 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGGTTGTGATGGGTCAGG |
| Suggested primer 2: | GCGAACCTCCCTCTTTACCC |