Insertion junction: CLIP2.013917_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.013917
Chromosome:chromosome 3
Location:5085532
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g180250 IPS1,INO1 (1 of 1) 5.5.1.4 - Inositol-3-phosphate synthase / Myo-inositol-1-phosphate synthase; Myo-inositol-1-phosphate synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCACAACACAGCCGCGGCTCGGTCGTCGCGTCGGCGGCAAGTGTGCGCT
Internal bar code:TAGTGGGGAGGGCAGTGTCCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:4361
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTGGCATGTTGCTCAAC
Suggested primer 2:CGAAGTCGGGGTCGTAGATG