Insertion junction: CLIP2.013917_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.013917
Chromosome:chromosome 3
Location:5085537
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g180250 IPS1,INO1 (1 of 1) 5.5.1.4 - Inositol-3-phosphate synthase / Myo-inositol-1-phosphate synthase; Myo-inositol-1-phosphate synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAACCCGGGGGGGCGCTGTAAGGGCTTCGCTTCAACTCTGCCTACCTC
Internal bar code:ACTACTGGTAGTTAATGATCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3027
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAGTCGGGGTCGTAGATG
Suggested primer 2:TGCTTGGCATGTTGCTCAAC