| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.013938 |
| Chromosome: | chromosome 6 |
| Location: | 2845181 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272100 | (1 of 1) K15463 - tRNA A64-2'-O-ribosylphosphate transferase [EC:2.4.2.-] (RIT1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACGGCAGTTGAAGAAGGAGCAACTGTCGCTCTACAACTGCCTGGCCT |
| Internal bar code: | TGATGTATGTTATGATATACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3823 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCAATCGACGTCGCAAC |
| Suggested primer 2: | CTCGAACGTGCGCACATAAG |