| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013951 |
| Chromosome: | chromosome 17 |
| Location: | 2444262 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715000 | HSP33 | Heat shock protein 33; (1 of 1) K04083 - molecular chaperone Hsp33 (hslO) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCAGGTGGGCGTGGTGGGAGGGGTGGAGTCACCTGATTCCGCGCAGGG |
| Internal bar code: | AATTGCGAGACCCCTCCATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1231 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCCGTGATCCTCGTTCC |
| Suggested primer 2: | AGACGCCAAACTACCACCTG |