Insertion junction: CLIP2.013959_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.013959
Chromosome:chromosome 17
Location:2771656
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g717950 PHC28,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 6 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTTGAGGTCAGCCTTGCAGCACTTGTGCGTGCAGCCAGCCGGCACCTT
Internal bar code:CTTACTAATTCTCCCTTCGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1400
LEAP-Seq percent confirming:77.1429
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:TAAAACTGCTAGGCGCACCA
Suggested primer 2:GGAGGAGAAGGAGGAGGAGG