| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013965 |
| Chromosome: | chromosome 6 |
| Location: | 3149413 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275800 | HTB31,HTB4,HTB3 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCTTGGCGGCCTTCTCGGCCTTGGGCTTGGCCTCAGCCTTGGCAGGA |
| Internal bar code: | GGACTCCTATACGGGGCTGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1443 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCAAACCCGTTCCCAAA |
| Suggested primer 2: | GTACACAGTACCACCCTGGC |