Insertion junction: CLIP2.013985_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.013985
Chromosome:chromosome 11
Location:917835
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467655 IMP1 Mitochondrial inner membrane signal peptidase; (1 of 1) K09647 - mitochondrial inner membrane protease subunit 1 (IMP1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGAGGCCACTGGGTTTGTTGTTCGCACCATCCCATGAGATAGCAAATCA
Internal bar code:TCCGTGTGATTCCGGGCACAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:800
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCAAAGGTTTCGCAGAGC
Suggested primer 2:GCGTATTTTGGGGGTGGAGA