Insertion junction: CLIP2.013987_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.013987
Chromosome:chromosome 17
Location:1567540
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g707200 LIC2 (1 of 5) PF02931 - Neurotransmitter-gated ion-channel ligand binding domain (Neur_chan_LBD); Ligand-gated ion channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTTTGCATCCCAGCATCTAACCTTTTTGTGTTTATCTTGTTTGGTACG
Internal bar code:CGCATAGTGGATGCTGGTGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1225
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TAGTCCACAGCGTGACCAAC
Suggested primer 2:TTCAGCACCTCGGAGAACAC