Insertion junction: CLIP2.013989_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.013989
Chromosome:chromosome 9
Location:1388263
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g400200 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCGCCCTCGCTGTGGTGGCGCTTGTCGCCGCTGCGCCGCGTGCCGTG
Internal bar code:TTTAATATCACACCGCGATTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3038
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:TACCACGAGGAAGACGACCT
Suggested primer 2:TCGTCGTCATCATCGTCGTC