| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013991 |
| Chromosome: | chromosome 6 |
| Location: | 3831965 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278177 | DNL6,LIG6 | (1 of 1) PTHR10459:SF44 - ANKYRIN REPEAT-CONTAINING PROTEIN; DNA ligase 6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGTAGCTGGAGTGCTCCGAGTAGGGCACCAGATGCACCGACAGCGCG |
| Internal bar code: | CCACGATTTTAATAGAAAGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1700 |
| LEAP-Seq percent confirming: | 90.7692 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCAGACGTGTTCACGGA |
| Suggested primer 2: | CCTCATCCGAGATCAGCACC |