Insertion junction: CLIP2.013995_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.013995
Chromosome:chromosome 15
Location:5583608
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre46.g760947 (1 of 7) IPR022772 - von Hippel-Lindau disease tumour suppressor, beta/alpha domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCCTCCTGCTCCTCCTGCTCCTCCTCTTCCTCGGGGTCGAAGTCGAAC
Internal bar code:TGGTGTTGTTTTTCTAGCACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:446
LEAP-Seq percent confirming:11.1111
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCATGTACCGCACCGTGA
Suggested primer 2:ATCTGAGCTTGTCCCTGCTG