| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.013999 |
| Chromosome: | chromosome 3 |
| Location: | 8457303 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g212641 | (1 of 16) 2.3.1.48 - Histone acetyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCCGGGTTCCAGCCCCCAAGTGGCACCTTAAGCCCCGCAGCACGGC |
| Internal bar code: | AGAAAAGAAGAATTTTTTAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2975 |
| LEAP-Seq percent confirming: | 94.8454 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCGCGGATTTGCATTCGT |
| Suggested primer 2: | GTCGTGGTGGTTGAGGATGT |