Insertion junction: CLIP2.014000_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.014000
Chromosome:chromosome 13
Location:1375579
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g571750 (1 of 1) PTHR10050 - DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAACCCAGTTGTCACCGCCGTCCGTCTGCGTGTCTGTGCCAAAGGCGCT
Internal bar code:ACCCCCAATAGAGTTGCGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1702
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGGCCGGAGATTTGAGTG
Suggested primer 2:TCGCAGTAGTGTCACCAACC