| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.014000 |
| Chromosome: | chromosome 13 |
| Location: | 1375619 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571750 | (1 of 1) PTHR10050 - DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGTATGTGGCAAAGGCGCGCGGGCCTCTCATCGCCAACCAGGCTGTCG |
| Internal bar code: | ACCCCCAATAGAGTTGCGAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3445 |
| LEAP-Seq percent confirming: | 99.4253 |
| LEAP-Seq n confirming: | 173 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 174 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCAGTAGTGTCACCAACC |
| Suggested primer 2: | AGTGGCCGGAGATTTGAGTG |