Insertion junction: CLIP2.014012_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.014012
Chromosome:chromosome 6
Location:86950
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g249500 MET1,DMC7,DMT1B,DMT1 (1 of 4) K00558 - DNA (cytosine-5)-methyltransferase 1 (DNMT1, dcm); Cytosine-C5 specific DNA methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATTCTCTGCACCCCGACCCAGTTCCGTGCTACAAAGCATTGACAGCACG
Internal bar code:TACCCTCCATTTGTAATTTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:4562
LEAP-Seq percent confirming:36.3636
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:56
LEAP-Seq n unique pos:88

Suggested primers for confirmation by PCR

Suggested primer 1:GCGATGATAAACACGCGCTT
Suggested primer 2:TGCTGTCGCATTGCACTAGA