Insertion junction: CLIP2.014020_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.014020
Chromosome:chromosome 16
Location:6124926
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g677200 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTGATGGATGTCGTGAGTGGCTGTCCCGTGGAGGTGCTGCAGCGGCA
Internal bar code:GGACGAAGCATCTTGCGTGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:4460
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGCAGGAAGGACAGCTTC
Suggested primer 2:AGGTGGCTTCGTCGCTTAAA