Insertion junction: CLIP2.014024_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.014024
Chromosome:chromosome 10
Location:6571930
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g465650 (1 of 2) IPR001680//IPR011047//IPR017986//IPR020472//IPR027417 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40-repeat-containing domain // G-protein beta WD-40 repeat // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGAGGCCAGCTCCATTTTGACTCAGCTGTCTTTCGCAATCCACAATC
Internal bar code:ACCGAGCAGGACTGCTAGCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:357
LEAP-Seq percent confirming:21.0526
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCCCGTCCACGTCACATC
Suggested primer 2:CACATACACAGCCCCCACAT